![]() KJ was supported by the Ohio State University Mayer’s Undergraduate Summer Research Fellowship and LW by the Systems and Integrative Biology Fellowship (NIH T32). This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: Array data are deposited at the Gene Expression Omnibus (GEO) NCBI database with accession number (GSE69607).įunding: This work was supported by funds from the International Foundation for Research on Paraplegia (P 129 to PP and MG), the National Institutes of Health (NIH R21 R21NS081413 to PP and MG and NIAID R01-AI092117 to SP-S), and the Ray W. Received: SeptemAccepted: DecemPublished: December 23, 2015Ĭopyright: © 2015 Jablonski et al. Olszewski, University of Michigan Health System, UNITED STATES (2015) Novel Markers to Delineate Murine M1 and M2 Macrophages. Overall, this work defines exclusive and common M1 and M2 signatures and provides novel and improved tools to distinguish M1 and M2 murine macrophages.Ĭitation: Jablonski KA, Amici SA, Webb LM, Ruiz-Rosado JdD, Popovich PG, Partida-Sanchez S, et al. In vivo, a similar CD38 + population greatly increased after LPS exposure. Conversely, CD38 labeled most (71%) in vitro M1 macrophages. Egr2 labeled more M2 macrophages (~70%) than the canonical M2 macrophage marker Arginase-1, which labels 24% of M2 macrophages. We further confirmed these data by flow cytometry and show that M1 and M2 macrophages can be distinguished by their relative expression of CD38 and Egr2. We validated by real-time PCR an M1-exclusive pattern of expression for CD38, G-protein coupled receptor 18 (Gpr18) and Formyl peptide receptor 2 (Fpr2) whereas Early growth response protein 2 (Egr2) and c-Myc were M2-exclusive. To provide a foundation for the complexity of in vivo macrophage phenotypes, we performed a comprehensive analysis of the transcriptional signature of murine M0, M1 and M2 macrophages and identified genes common or exclusive to either subset. It has been difficult to dissect macrophage phenotypes in vivo, where a spectrum of macrophage phenotypes exists, and also in vitro, where low or non-selective M2 marker protein expression is observed. Classically (M1) and alternatively activated (M2) macrophages exhibit distinct phenotypes and functions.
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